Technical specifications

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General features

Excitation Pulsed Nitrogen Pumped Dye Laser with Exchangeable Dye Modules
337 nm - 785 nm
Detection Photomultiplier
1 or 2 Measuring Channels
Exchangeable Emission Filters
350 nm - 900 nm
Microplate formats 6 - 1536
Dimensions 45 x 82 x 52 cm (W x D x H)
Software MikroWin 2000 with Automatic Deconvolution Function

Filter selection

A number of combinations of laser modules providing different excitation wavelengths with selected detection filters provides the highly sensitive detection of the fluorescence signal of nearly all common fluorescence labels. - See "Filter-Selection Guide"

Type-dependent specifications

 

LF 500 NanoScan FLT

LF 500 NanoScan HT

Measuring Method FI
Nano-TRF
FLT
TRF (option)
FI
Nano-TRF
FLT
TRF (option)
Detection Limit
(Cy5 in black 384 well plate)
2 pM 2 pM
Dynamic Range 105 105
Measuring Range in Nano-TRF Mode 0 ns - 20,000 ns 0 ns - 20,000 ns
Measuring Range in FLT mode 0 ns - 20,000 ns
0 ns - 1,000,000 ns (option)
0 ns - 20,000 ns
0 ns - 1,000,000 ns (option)
FLT Precision 1.5% at 4 ns 1.5% at 4 ns
Time Resolution 100 ps 50 ps
Measuring Range in TRF Mode 0 ns - 1,000,000 ns (option) 0 ns - 1,000,000 ns (option)
Measurement Time
96 well plate
384 well plate
2:00 min
7:10 min
1:40 min
5:00 min