LF 500 NanoScan

   Overview   |   Principles of Operation   |   Technical Specifications   |   Applications

Multi-functional Reader for Microplates with Fluorescence Lifetime Resolution

Fluorescence labels have well-known properties such as absorption and emission maximum, extinction and quantum yield. They also display another characteristic feature:

fluorescence lifetime

This molecule-specific constant is particularly useful for robust homogeneous assay platforms.  The examples below list some of the important properties of a few selected fluorophores.

Application Examples

  • molecular binding assays
  • protein-protein interaction
  • enzyme activity
  • enzyme kinetics
  • rezeptor-ligand binding
  • DNA hybridisation
  • calcium determination
  • cell-based assays

Literature

Technical Documents

Examples

Hybridization of a 15-mer Oligonucleotide (Enlarge)


Estrogen Assay (Enlarge)


Attachment of a Dye Molecule to a Protein (BSA) (Enlarge)


Readout of Oxygen Sensitive Microplates via Lifetime (Enlarge)