LF 401 Lambda Fluorescence Spectrometer

Compact Fibre-optic Fluorescence Spectrometer with Nanosecond Time-resolution

   Overview   |   Principles of Operation   |   Technical Specifications   |   Applications

Applications

Characterisation of fluorescing samples

• Characterisation of bound and unbound dye moelcules
• Characterisation of semiconductors
• Characterisation of oils
• Investigation of excimer formation
• Investigation of surfaces
• Investigation of thin layers and films
• Identification of pollutants

Online process monitoring

• Identification of pollutants
• Fluorescence lifetime based fibre-optic sensor technology
• Bio-technologic cell culture
• Interaction of active agents with biologic systems
• Ageing processes in fruit and vegetables
• Waste-water monitoring
• Environmental monitoring (e.g. PAK)

Application example 1: time-resolved spectra of mixed aqueous NADH and flavine solutions (Concentrations: NADH: 1.8*10^-5 M; Riboflavin: 6.3*10^-6 M)

Examples

Application Example 1

Width of electronic gate: 2 ns Position of electronic gate: 0 ns Both fluorescence maxima at 465 nm and 525 nm can clearly be identified Width of electronic gate: 2 ns position of electronic gate: 4 ns Width of electronic gate: 2 ns position of electronic gate: 8 ns only long-lasting riboflavine fluorescence is detected because of changed gate position

Application Example 2

3-D-Spectrum of Rhine water with added fluorescent dyes
(Fluorescence intensity as function of emission wavelength and time)

Rhine water fluorescence was excited with nitrogen laser pulses of 350 ps duration at 337 nm. The first spectral maximum at 381 nm is related to the Raman band of water. The fluorescence band at about 450 nm results from "yellow matter" that occurs naturally in surface water and that derives from leaves degradation. Another fluorescence band at 510 nm is related to Uranin (a fluorescein derivative).